Discovery and Analysis of Alginate-Degrading Bacteria from
South Korea Territory

(우리나라 영토의 알긴산 분해 박테리아의 발견 및 분석)

Gil Dong Hong1*, Sun Shin Lee1,2

1 Division editorial department of the Korean Society of Environmental Agriculture, Seoul 00000, Korea

2 Korea University Agricultural Research Institute, Seoul 00000, Korea

E-mail: 123456@korea.kr

홍길동1*, 이순신1,2

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Alginate is a high-molecular carbohydrate produced by seaweed or some bacteria, and by itself, it is used as a food and pharmaceutical material, but alginate oligosaccharides(AOs) produced by various alginate lyases are known to have various biocontrol functions, including anticancer, immune enhancement, and anticoagulant action. Alginate lyases are found in soil or marine microorganisms, and in the intestines of marine animals. In this paper, 13 species of alginate-degrading bacteria were isolated from beach soil bacteria in South Korea using 3,5-Dinitrosalicylic acid (DNS) and their alginate-degrading properties were investigated. In the 16s rDNA sequencing analysis, Marinomonas polaris, Zobella aerophila, and Pseudomonas benzenivorans were classified as new bacteria at the subspecies level, and in this report, one of them, Zobella aerophila, revealed alginate-degrading properties for the first time. Using five bacteria with relatively high-degradative activity, the substrate specificity of each bacterial enzyme for the two major polymer linkages found in alginate, poly-guluronate or poly-mannuronate, was investigated and the location of its degradation properties was identified. All bacteria exhibited high substrate specificity for polyM, and were shown to secrete the enzyme out of the cell. In addition, the biofilm formation properties and Auxin biosynthesis ability of the isolated bacteria were also identified by Biofilm biomass formation assay kit and Salkowski test, respectively. Six strains exhibited moderate or strong biofilm formation characteristics, and three strains were identified to biosynthesize IAA. After that, the amino acid sequence of Alginate lyase was obtained through the whole genome sequencing analysis to predict the properties of the enzyme. The results of this report provide useful data and information for various industrial fields such as pharmaceuticals, energy, food, and beauty for generation AOS using alginate lyase.

Keywords: Alginate, Alginate-degrading bacteria, Alginate lyase, Seaweed